Determination of Concentrations of Tacrolimus in Whole Blood With ELISA and Its Application
- VernacularTitle:酶联免疫吸附检测法检测全血中他克莫司浓度及其应用
- Author:
Mingxiong ZHANG
;
Jian HUANG
;
Zhiling DAI
;
Lizhen QIU
;
Lan CUI
- Publication Type:Journal Article
- From:
China Pharmacy
2001;12(4):225-226
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To discuss the accuracy of detecting tacrolimus(FK506) concentration in whole blood by ELISA and its application in organ transplantation.METHODS:The ELISA was used to detect FK506 valley point concentrations in 5 liver transplanted recipients at different postoperative periods .Based on the results combined with clinical findings, the dosages were adjusted.The accuracy of the method and guidance action in FK506 clinical application were described.RESULTS: In 131 detections of 350 samples, the results of quality control samples were all in the control range .The detecting RSDs of high and low concentration quality control samples were 9.09% and 11.90%, respectively.The control range of FK506 concentrations in whole blood were similar to that reported in literature .CONCLUSION :ELISA is a sensitive and specific method for routine FK506 concentration monitoring in whole blood,which is suitable for application in hospital.
