Determination of Allitridum in Human Serum by RP - HPLC
- VernacularTitle:反相高效液相色谱法测定大蒜素的血药浓度
- Author:
Yalin DONG
;
Xianmin WANG
;
Haiyan DONG
- Publication Type:Journal Article
- From:
China Pharmacy
2001;12(4):226-227
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To develope a reversed-phase high performance liquid chromatographic(RP-HPLC) method for the determination of allitridum in human serum.METHODS: The serum sample was injected after being deproteinized with trichloroaceticacid and being extracted with hexane.A Phenomenex C1s was used as the stationary phase and the solution being composed of acetonitrile- 1%HAc(62: 38,V/V)as the mobile phase with a flow rate of 1.2 ml/min .The serum samples were monitored at 240nm.RESULTS: The peak area of allitridum against allitridum concentration was linear with a correlation coefficient of 0.9 977 over the range of 0.29～12.4 μg/ml, the limitation of detection was 0.18 μg/ml.The average recovery was (99.2 ± 6.6) %, within - day RSD＜ 5.5% (n = 5), between - day RSD＜ 5.8% (n = 5) .CONCLUSIONS: This method is rapid, accurate and sensitive,it is very suitable for the investigation on clinical pharmacokinetics of allitridum.
