Study on targetability of asialofetuin-linked liposomes to he patocytes in mice
- VernacularTitle:无唾液酸胎球蛋白修饰脂质体在小鼠体内的肝实质细胞靶向性研究
- Author:
Li WANG
;
Guiming SHU
;
Xiangtao WANG
;
Sha LI
;
Xinpu HOU
- Publication Type:Journal Article
- From:
Journal of Peking University(Health Sciences)
2001;33(3):251-254
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the possibility of liposomes to ta rget hepatocytes af ter being linked with asialofetuin(AF). Methods: The biodistribu tions in differen t cells in liver as well as in blood, in various organs such as heart, spleen, l ung and kidney of mice, of traditional sterically stabilized liposomes(SSL) , AF-linked normal liposomes(AF-NL) and AF-linked sterically stabilized lipos ome s(AF-SSL) were studied by radioisotopic labeling. Results: The half-live s of SSL, AF-NL and AF-SSL were 14.44, 4.73 and 11.49 h， respectively. The di strib ution of liposomes in liver was AF-NL>AF-SSL>SSL. There was significant differenc e among these three formulations. In hepatocytes and non-hepatocytes, the conce ntr ations of the liposomes were both in line with the sequence AF-NL>AF-SSL>SSL( P <0.05). However, the ra tios of the concentrations in hepatocytes to that of non-hepatocytes were AF-N L≈AF -SSLSSL( P <0.05). Conclusion: After being labeled with asi alofetuin, liposomes can target hepatocytes very we ll， whether in long-circulation or not.
