Down regulation of NMDA receptor subunits NR2A and NR2B mRNA e xpression after audiogenic seizure in epilepsy-prone rat brain
- VernacularTitle:听源性惊厥刺激使P77PMC大鼠脑内NR2A/2B基因表达下调
- Author:
Yuwu JIANG
;
Weisong SHAN
;
Xiru WU
- Publication Type:Journal Article
- From:
Journal of Peking University(Health Sciences)
2001;33(3):266-269
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the expression of N-methl- D -aspartate (NMDA) receptor subunits NR2A and N R2B mRNA after audiogenic seizure in the brain of genetically epilepsy-prone ra t s-P77PMC. Methods: In situ hybridization technique was used to stud y the mRNA expre ssion of NR2A and NR2B at different times after audiogenic seizure in different b rain areas of P77PMC rats. Results: The NR2A mRNA expression showed time-depende nt down-regulation in cerebral motor and sensory cortex, hippocampus CA1-CA3, de ntate gyrus after audiogenic seizure. Down-regulation emerged 30 min after se izure, 2 hours later the mRNA reached the lowest level， 4-8 hours later re turned to the baseline, and 24 hours later decreased lower than baseline again . The mRNA expression of NR2B was quite similar to that of the NR2A af ter seizure. Conclusion: The down-regulation of NR2A and NR2B mRNA indicates that the subunit composition of the NMDA receptor complexes may be altered after aud iogenic seizure. These structural alterations could make the function of NMDA re ceptor complexes change and subsequently influence the excitability of brain as well as the susceptibility of epilepsy.
