Determination of astragaloside in serum with anisic acid by spectrophotofluorimetry
- VernacularTitle:茴香酸荧光分析法测定血清中黄芪甲甙
- Author:
Ming DU
;
Yangqing LIU
;
Zhi YAN
;
Bingjiu XU
- Publication Type:Journal Article
- From:
Journal of Peking University(Health Sciences)
2001;33(3):274-276
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop a new determination method of astrogaloside in s erum by spectrophotofluorimetry. Methods: The fluorescence char acteristics of astragaloside anisic reaction product were used to determine astragaloside in serum. Re sults: The reaction product of astrogaloside and anisic acid had excitatio n and emission maxima were at 320 and 387 nm, respectively. The linear range of determ inati on was between 0.2 and 40.0 mg*L －1. The detection limit was 0.02 mg*L －1. The recovery of measurement was between 98.8% and 102.4%. Conclusion: The propos ed method has advantages of high sensitivity, low detection limit and no interference. It has been used to determine astragaloside in serum successfully.
