Isolation, culture and characterization of cochlea microvascular endothelial cells from guinea pigs
- VernacularTitle:豚鼠耳蜗微血管内皮细胞的分离培养与鉴定
- Author:
Yunjun WEI
;
Xueyuan ZHANG
- Publication Type:Journal Article
- From:Journal of Third Military Medical University
2001;23(3):324-236
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up a reliable method for isolating and culturing cochlea microvascular endothelial cells. Methods Cochlea stria was separated by micrergy from guinea pigs and the stria tissue nubbles were cultured in vitro. Results Two days later, a few cells were seen disseminately around partial tissue nubbles and their number was increased with time prolongation. There were some cellular colonies consisted of large quantity of cells around the partial tissue nubbles on 10 th day. Under the inversion microscope, the single cultured cells presented a long fusiform and the confluent monolayer cells linked tightly and exhibited the typical structure of “scree” of cultured endothelial cells in vitro. After purification, over 95% of the cultured cells showed factorⅧ relative antigen positive reaction. Conclusion The method used in this study can obtain cochlea microvascalar endothelial cells of guinea pig in vitro.
