EFFECT OF LIDAMYCIN ON THE EXPRESSION OF GENES INVOLVED IN INVASION REGULATION IN HCT-8 HUMAN COLON CANCER CELLS
- VernacularTitle:力达霉素对人结肠癌HCT-8细胞侵袭调节基因表达的影响
- Author:
Dapeng CUI
;
Zhen WANG
;
Diandong LI
- Publication Type:Journal Article
- From:
Acta Pharmaceutica Sinica
2001;36(4):246-249
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the effect of lidamycin on the expression of genes involved in invasion regulation in HCT-8 human colon cancer cells. METHODS HCT-8 human colon cancer cells were treated with lidamycin (10 nmol*L-1) for 8 h. The effect of lidamycin on the expression of genes were detected by cDNA arrays, Northern blot and RT-PCR. RESULTS Hybridization of the entire cDNA populations to Atlas Arrays showed that lidamycin down-regulated the expression level of MMP-9 and up-regulated the expression level of TIMP-1. These changes were confirmed by Northern blot and RT-PCR. CONCLUSION The results indicate that lidamycin may exhibit its anti-invasive activity by inhibitting the production of type IV collagenase whilst enhancing the production of tissue inhibitor metalloproteinase.
