Determination of content of astragaloside A in Astragalus membranaceus powder for
- VernacularTitle:薄层色谱-分光光度法测定黄芪粉针剂中黄芪甲苷的含量
- Author:
Chunxin YANG
;
Lixia SUN
;
Genying XU
- Publication Type:Journal Article
- Keywords:
Astragalus membranaceus (Fisch.) Bge. powder for injection (AMPI);
TLC-spectrophotometry;
astragaloside A
- From:
Chinese Traditional and Herbal Drugs
2001;32(4):312-314
- CountryChina
- Language:Chinese
-
Abstract:
To develop a method for the quality control of Astrgalus membranaceus (Fisch.) Bge. powder for injection (AMPI). Methods TLC-spectrophotometry was used to determine the content of astragaloside A in AMPI. The mobile phase was chloroform-methanol-water (65∶30∶10) and detected at the wave length of 418 nm. Results The regression equation within the spotted range of 80~240 μg was A=1.538 C+ 0.189, with good linearity, r=0.999 8. The average recovery rate was 98.68% and RSD=2.52%(n=5). Conclusion The method used simple and easily available instruments and the rusults obtained were reliable and specific, suitable for the quality control of AMPI.