Distinction between Gastrodia elata and its four falsified specimens and determination of gastrodin by capillary electrophoresis
- VernacularTitle:天麻及其伪品的毛细管电泳鉴别及天麻素的测定
- Author:
Dexian WANG
;
Gengliang YANG
;
Haiguang LIU
;
Yunxia YANG
- Publication Type:Journal Article
- Keywords:
capillary electrophoresis;
Gastrodia elata Bl.;
gastrodin
- From:
Chinese Traditional and Herbal Drugs
2001;32(4):351-352
- CountryChina
- Language:Chinese
-
Abstract:
To develop a method for the distinction of genuine Gastrodia elata B1. from its four falsified plants of common occurrence and determination of its active principle, gastrodin by capillary electrophoresis. Methods The electrophoretic conditions were a buffer solution containing 24 mmol/L borate (pH=9.4), at a voltage of 16 kV. Results The electrophorograms obtained under the above conditions showed easily distinguishable differences. Baseline separation of gastrodin was achieved and its quantitative analysis can be accomplished within 15 min. The linear correlation equation was Y=-0.048+7.79X (r=0.999). Conclusion This method could serve as an easy and precise method for the quality control of G. elata.
