ISOLATION AND IDENTIFICATION OF ATRAZINE-DEGRADING STRAINS
- VernacularTitle:阿特拉津降解菌株的分离和鉴定
- Author:
Baoli CAI
;
Jinyong HUANG
;
Jiandang SHI
;
Xinping ZHANG
;
Hai LIU
;
Changshou ZHU
- Publication Type:Journal Article
- From:
Microbiology
2001;28(2):22-26
- CountryChina
- Language:Chinese
-
Abstract:
Six atrazine-degrading strains, Pseudomonas spp. AD1, AD2, AD6, Agrobacterium sp. AD4, Xanthomonas AD5, and Erwinia sp. AD7, were isolated from industrial wastewater. These strains are able to grow on atrazine as sole nitrogen source. Strain AD1 is able to degrade atrazine of 0. 3g/L in minimal medium at a percentage of 99.9% in 72 hours. PCR products that are homologous to the atrazine chlorohydrolase gene atzA)from Pseudomonas sp. strain ADP were obtained by PCR method using total DNA of the strains AD1 ,AD4,AD5,AD6,and AD7 as templates.