Alterations of surfactant protein A and B in the early stage of acute lung injury in rats
- VernacularTitle:急性肺损伤早期肺表面活性蛋白A、B含量的变化
- Author:
Yan XIAO
;
Shehuai CUI
- Publication Type:Journal Article
- From:Journal of Third Military Medical University
2001;23(2):154-156
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the alterations of surfactant protein A (SP-A) and B (SP-B) in rats in the early stage of acute lung injury induced by oleic acid. Methods An acute lung injury model was established by injecting oleic acid into the superior vena cava of rats and the levels of SP-A and SP-B in branchoalveolar lavage fluid (BALF) were determined with Western Blot and ELISA. Results The levels of SP-A and SP-B in BALF were significantly decreased in the injured group 1 h after acute lung injury (P<0.01) and remained low in the next 6 h. Conclusion The levels of SP-A and SP-B were decreased in the early stage of acute lung injury. The methods used for detecting SP-A and SP-B we adopted are specific and sensitive, and might be helpful for other experimental study.
