Concentration of Ca2+, contents of cAMP, CaM and Ca2+/CaM-PK II in pheochro-mocytoma PC12 cells after combined soman and hypoxia injury
- VernacularTitle:缺氧复合梭曼中毒PC12细胞中［Ca2+］、cAMP、CaM和Ca2+/CaM-PK Ⅱ的变化
- Author:
Jiqing ZHAO
;
Qiang WU
;
Shili WANG
;
Xiangde WEI
;
Zhaojun DONG
;
Yunpeng LI
;
Yong LIU
- Publication Type:Journal Article
- From:Journal of Third Military Medical University
2001;23(2):169-171
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes of the concentration of Ca2+, contents of cAMP, CaM and activity of Ca2+/CaM-PK II in pheochromocytoma PC12 cells after combined soman and hypoxia injury. Methods The changes of ［Ca2+］, and activity of CaM, cAMP and Ca2+/CaM-PK II in PC12 cells were studied after combined soman and hypoxia injury with radioimmunoassay. Results The changes of ［Ca2+］, the contents of CaM, cAMP were significantly higher in hypoxic and soman intoxicated group than in soman intoxicated group and control group under hypoxia; but the activity of Ca2+/CaM-PK Ⅱ were significantly decreased. Conclusion ［Ca2+］, CaM, cAMP and Ca2+/CaM-PK Ⅱ exert important role in the damage of PC12 after combined soman and hypoxia injury.
