Effects of sin-1 on growth and cytoskeleton of endothelial cells in vitro
- VernacularTitle:Sin-1对内皮细胞生长及细胞骨架的影响
- Author:
Yunlai LIU
;
Lunshan XU
;
Zhongxiang YAO
;
Wenqin CAI
- Publication Type:Journal Article
- From:Journal of Third Military Medical University
2001;23(4):422-424
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism affecting on permeability of vascular endothelial cell by nitric oxide (NO). Methods Series concentration of sin-1（a donor of NO） were added to ECV 304, a cell line of human umbilical vein endothelium. Cell growth and expression of f-actin, a cytoskeleton protein were observed. Results Cell growth was inhibited with a dose from 6.25 to 100 μmol/L and was caused to death at the concentration of 50 to 100 μmol/L by sin-1. The expression of f-actin was suppressed obviously after cultured with 100 μmol/L sin-1 for 4 hours. Conclusion It suggests that anomaly increased NO can increase permeability of blood vessels by suppressing the expression of f-actin, inhibiting cell growth or even resulting in cell death.
