he roles of hypoxia and glutamic acid on NMDA receptor channel of hypothalamic neuron in rats
- VernacularTitle:缺氧及谷氨酸对大鼠下丘脑神经元NMDA通道的影响
- Author:
Penghui CHEN
;
Huaizhen RUAN
;
Xigui WU
- Publication Type:Journal Article
- From:Journal of Third Military Medical University
2001;23(4):429-431
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of hypoxia and glutamic acid on the kinetic properties of NMDA receptor channel of the hypothalamic neurons in rats. Methods Cell-attached mode patch clamp technique was employed to record the single channel current of the NMDA receptor. Results The open probability of NMDA receptor channel was increased after acute hypoxia compared with that of normal state, the open time τ1,τ2 was changed from (0.33±0.10)ms，(4.36±0.26)ms to (0.93±0.22)ms，(7.64±0.72)ms, and the close time τ1,τ2 was from (18.03±3.50)ms，(171.50±19.10)ms to (3.42±1.02)ms，(19.39±3.07)ms. The mean open probability was changed from 0.12±0.05 in normal state to 0.66±0.36 in hypoxia state. Furthermore, glutamic acid can increase open time and open probability of NMDA receptor channel,decrease close time. Conclusion The excitability and the open probability of NMDA receptor channel of hypothalamic neurons increased by hypoxia is related to glutamate.
