Resolution of Racemic Thyroxine with Chiral Additive asMobile Phase by High Performance Liquid Chromatography
- VernacularTitle:高效液相色谱手性流动相法拆分甲状腺素对映体
- Author:
Rong WANG
;
Jingwen XIE
;
Zhengping JIA
;
Xiaoli HU
;
Liren CHEN
- Publication Type:Journal Article
- From:
Chinese Journal of Analytical Chemistry
2001;29(4):406-409
- CountryChina
- Language:Chinese
-
Abstract:
A high performance liquid chromatographic (HPLC) method using triethylamine (TEA), copper *" acetate and L-proline as the chiral mobile phase (CMP) and the enantiomric analysis of thyroxine (T4) are described. The influence of the mobile phase parameters including the TEA concentration, manipulation pH, concentration of Cu*", concentration of L-proline, temperature of column and flow rate of eluent on the enantiomers separation of thyroxine was studied. The HPLC-CMP is a simple method for separation and determination of D，L-T4 in drug or in human plasma. It can be used to therapeutic drug or adverse drug reactions monitor.
