Capillary Electrophoresis——An Attractive Alternative Tool for Analyses ofGenetic Mutations/Polymorphisms
- VernacularTitle:毛细管电泳——基因突变及多态性分析新方法
- Author:
Jicun REN
- Publication Type:Journal Article
- From:
Chinese Journal of Analytical Chemistry
2001;29(4):461-465
- CountryChina
- Language:Chinese
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Abstract:
Recently, capillary electrophoresis (CE) has been successfully used for genetic analysis instead of conventional slab gel electrophoresis. This article will give an overview of the fundamental aspects on mutation/polymorphism analyses in combination with capillary electrophoresis (CE), which mainly includes single strand conformation polymorphism analysis, denaturing gradient-gel electrophoresis, heteroduplex analysis, chemical mismatch cleavage, restriction fragment length polymorphism, allele specific oligonucleotide hybridization, allele specific amplification, primer extension, and minisatellite and microsatellite analyses. A number of key applications are summarized.
