Determination of Concentration of Fentanyl in Human Plasma by HPLC With UV Detection
- VernacularTitle:HPLC－紫外法测定人血浆中芬太尼浓度
- Author:
Changfu LIN
;
Chunting LIU
;
Wenzhi LI
;
Enyou LI
- Publication Type:Journal Article
- From:
China Pharmacy
2001;12(5):286-288
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To determine the concentration of fentanyl in human plasma by HPLC with ultraviolet detection METHODS： External standard method was used The eluation of fentanyl was carried out by shim－ Pack CLC－ ODS（ 6 0mm× 150mm， 5μ m） column with 0 015mol/L NaH2PO4 acetonitril－ H2O（ 30∶ 70， v/v） as the mobile phase， and detection wavelength was 195nm RESULTS： Calibration curve for fentanyl was linear in the range of 2 0～ 100ng/ml（ r=0 999） The detection limit was 1 0ng/ml The within－ day RSD was （ 6 50± 2 79） ％ ， the between－ day RSD Was （ 6 70± 3 04） ％ The method recovery was （ 91 70± 4 70） ％ The extraction recovery was （ 97 38± 3 69） ％ CONCLUSION： This method was quick and accurate for the determination of fentanyl in human plasma and useful for pharmakinetic study of fentanyl
