Effects of 6， 7－ dimethoxycoumarins on Cell Proliferation and Cell Generation Cycle of Human Lung Car_cinoma Cell in Vitro
- VernacularTitle:茵陈素对肺癌细胞增殖和细胞周期的影响
- Author:
Yunzhong TAN
;
Guohua DAI
;
Jun LI
- Publication Type:Journal Article
- From:
China Pharmacy
2001;12(5):267-268
- CountryChina
- Language:Chinese
-
Abstract:
AIM： To investigate the effects of 6， 7－ dimethoxycoumarins on the proliferation and the cell cycle of human lung carcinoma cell（ PA） in vitro METHODS： The effects of 6， 7－ dimethoxycoumarins on the cytomorphology， growth and cell cycle of PA cells were evaluated by MTT assay and flow cytometry in vitro RESULTS： 6， 7－ dimethoxycoumarins could inhibit the proliferation of PA cells in a dose－ dependent manner The inhibition action of 6， 7－ dimethoxycoumarins plateaued at 160μ g/ml(52 4％ inhibition) The agent in dose of 80μ g/ml led to a decline in the proportion of cells to those in G2/M phase， made the cells remain in G0/G1 phase and reduced the proliferation index(PI) of PA cells CONCLUSION： In vitro， 6， 7－ dimethoxycoumarins can inhibit the proliferation of PA cells， which leads to arrest of the cells in G0/G1 phase by way of inhibiting DNA synthesis
