Transfecting and killing effect of herpes simplex virus thymidine kinase (HSV-TK) gene on human gastric carcinoma in vitro
- VernacularTitle:逆转录病毒介导的HSV-TK基因系统对人胃癌细胞的转染及杀伤作用逆转录病毒介导的HSV-TK基因系统对人胃癌细胞的转染及杀伤作用
- Author:
Yi WANG
;
Zhiqian HU
;
Xuejun XU
;
Yuanhe WANG
;
Qiang WANG
;
Xiantao KONG
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2001;22(4):328-330
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study retrovirus (RV)-mediated transduction of gastric carcinoma cells with the herpes simplex virus thymidine kinase (HSV-tk) gene and the subsequent treatment with ganciclovir(GCV). Methods: The TK gene was transfected into human gastric carcinoma cell line MKN28 using HSV-TK that packed with PA317 cell, the sensitivity of MKN28TK cells to GCV was examined in vitro. Results: The retroviral-mediated HSV-TK gene can be transfected to MKN28 cells. The growth rate of MKN28 cells transfected with HSV-TK gene did not change. MKN28TK cells became significantly sensitive to GCV and had bystander effect. Conclusion: Transfection of gastric carcinoma with HSV-TK has higher transfection efficiency. MKN28TK cells are significantly sensitive to GCV.
