Preliminary study of the lethal effects of E. coli DNA on mice
- VernacularTitle:大肠杆菌DNA对小鼠致死作用的初步研究
- Author:
Wendong PAN
;
Hong ZHOU
;
Jiang ZHENG
;
Peiyuan XIA
;
Xiaojian QIN
;
Yongling LU
;
Xiaolu LIU
- Publication Type:Journal Article
- From:Journal of Third Military Medical University
2001;23(4):395-397
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of bacterial DNA in systemic inflammatory response syndrome (SIRS). Methods A total of 100 mice of Kunming species were divided into ten groups: E.coli DNA (30, 20, 10, 5 and 1 mg/kg ), 30 mg/kg of CT DNA, 60Co DNA, DNased DNA, organic residue of DNA extraction and sterile water control. The last two were pre-treated with D-galactoamine (600 mg/kg intra peritoneally). Animals were administratively injected via tail vein. General physical condition and the death rate of mice were observed within 48 h. Results ①Obvious lethal effect of double strand E.coli DNA on mice were observed with a dose-effect correlation, LD50=11.51 mg/kg. ②NO difference in death rate was found in the group of 30 mg/kg E.coli DNA with or without 60Co irradiation (10/10 and 8/10,P>0.05). ③No rats died in the group of DNased DNA, organic residue of DNA extraction and calf thymic DNA (0/10). Conclusion Bacterial DNA may play an important role in the development of SIRS.
