polymorphism detection of asthms related genes
- VernacularTitle:支气管哮喘遗传相关基因的研究
- Author:
Limin IANG
;
Huiru ZHU
;
Xiaodong ZHANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2001;5(5):95-96
- CountryChina
- Language:Chinese
-
Abstract:
Objectiveto detect polymorphism of asthma related genes. Methods Patients diagnosed asthma were divided into twogroups , one group has genetic susceptibility , the other has no such character. DNA were extracted from the peripheral blood, then TG se-quence in second intron of IL-4 was amplified by PCR, the polymorphism of that segment were then detected through 8% po]yacrylamidegel. Resultfour kinds of penotype were found in such segment. The frequency of phenotype 1 and type 2 of in test group are statisticallydifferent from that in the check group. But the other two phenotype frequencies are just the same in two groups. Conclusionthe differentfrequency of phenotype I and phenotype 2 in two groups may give us some meaning in asthma genetics.
