Human mesenchymal stem cells differentiate into neuron-like cells
- VernacularTitle:成人骨髓间质干细胞定向诱导为神经元样细胞的研究
- Author:
Peng XIANG
;
Wenjie XIA
;
Xiuming ZHANG
;
Yan LI
;
Shunong LI
;
Lirong ZHANG
;
Zhenguang CHEN
- Publication Type:Journal Article
- From:
Chinese Journal of Pathophysiology
2001;17(5):385-387
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the differentiation from human mesenchymal stem cells(hMSC) into neuron-like cells.METHODS:hMSC were separated from rib marrow with Ficoll-Paque reagent and expanded in culture medium. hMSC were induced to differentiate with DMEM/monothioglycerol or DMEM/β-mercaptoethanol, respectively. Neuron-specific enolase(NSE), neurofilament(NF), and glial fibrillary acidic protein(GFAP) were detected by immunohistochemistry. RESULTS:hMSC were expanded as undifferentiated cells in culture for more than 5 passages. When treated with monothioglycerol or β-mercaptoethanol for 5 hours, hMSC exhibited neuronal phenotype. The expression of NSE and NF in the neuron-like cells was positive, but the glial astrocyte marker GFAP didn't express. CONCLUSION:hMSC can be induced to differentiate into neurous.
