Role of nuclear factor κB on the expression of interleukin-6 in mouse mesangial cells induced by interleukin-1β
- VernacularTitle:NF-κB活化在IL-1β诱导的小鼠肾小球系膜细胞表达IL-6中的作用
- Author:
Hanshi XU
;
Rengao YE
;
Qiongqiong YANG
;
Lin SUN
;
Niansheng YANG
;
Youji LI
;
Lixia ZENG
- Publication Type:Journal Article
- From:
Chinese Journal of Pathophysiology
2001;17(5):428-430
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the regulatory role of nuclear factor κB (NF-κB) in the expression of interleukin-6 in mesangial cells (MC) induced by interleukin-1β.METHODS:Activation of NF-κB was measured by electrophoresis mobility shift assay (EMSA). RT/PCR and ELISA were used to detect IL-6 mRNA expression and IL-6 production, respectively.RESULTS:rhIL-1β could rapidly stimulate the activation of NF-κB in MC, and increase the expression of IL-6 mRNA and protein. PDTC, one of the inhibitor of NF-κB， could inhibit the expression of IL-6 in mRNA and protein in MC stimulated by rhIL-1β.CONCLUSION:IL-6 expression induced by IL-1β may be regulated by NF-κB in MC, NF-κB may modulate the immune-inflammatory reaction in glomerular disease.
