Generation and characterization of a series of monoclonal antibodies against recombinant human augmenter of liver regeneration
- VernacularTitle:一组抗重组人肝再生增强因子单克隆抗体的制备与鉴定
- Author:
Lianping YANG
;
Minghua TONG
;
Xuerui YI
;
Huayin WAN
;
Pinglu ZENG
;
Xiangping KONG
;
Zhongyuan SHAN
- Publication Type:Journal Article
- From:
Chinese Journal of Pathophysiology
2001;17(5):441-443
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To generate monoclonal antibodies against human augmenter of liver regeneration (rhALR). METHODS:After BALB/C mice were immunized by the purified rhALR, the cells of spleen were fused with the cells of SP2/0; The titer and speciality were respectively fathomed from ascites or foster fluid by ELISA and Western-blot test. RESULTS:2 hybridoma cell lines were successfully obtained. The McAbs titer from ascites and foster fluid are respectively about 10-3-10-5 and 10-2-10-3. It is evident that the two McAbs were directed at different epitopes. CONCLUSIONS:The McAbs have higher speciality. It is significantly useful of the value that how hALR distribute in tissue organs, how the hALR signals the metabolism in the body and the control distribution of the hALR on cell growth on the translational level and so on is researched.
