Cultivation and isolation of the bone marrow mesenchymal stem cells
- VernacularTitle:骨髓间充质干细胞的分离与培养
- Author:
Guoping AI
;
Yongping SU
;
Guohe YAN
;
Xingze RAN
;
Xiaohong LIU
;
Chengji LUO
;
Tianmin CHENG
- Publication Type:Journal Article
- From:Journal of Third Military Medical University
2001;23(5):553-555
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe some biological features of bone marrow mesenchymal stem cells and explore the best conditions for isolatin g and culturing in vitro. Methods Common cell culture techn ique, light and electron microscopy were used to study the effects of the growth , proliferation, morphology of the bone marrow mesenchymal stem cells in differe nt adherent time, concentration of serum and cell density. Results The best culture condition in vitro for growth was 4-24 hours adherent time, 5%-10% fetal bovine serum, (4-8)×104/ml cell density. The cells were sp indle in shape and had a strong ability of proliferation. The time for cell duplication was 3 to 4 days. The cells showed the characteristics of stem cell s in electron microscope. Conclusion The best condition for iso lation and culture of bone marrow mesemchymal stem cells was successfully establ ished and some biological features were obserred. It found a base for further in vestigation and using of mesenchymal stem cells.
