Monitoring the Process of Bovine Insulin Unfolding by Capillary Zone Electrophoresis
- VernacularTitle:毛细管区带电泳监测牛胰岛素的去折叠过程
- Author:
Biao DONG
;
Fangting DONG
;
Jinglan WANG
;
Xiaohong QIAN
- Publication Type:Journal Article
- From:
Chinese Journal of Analytical Chemistry
2001;29(5):538-541
- CountryChina
- Language:Chinese
-
Abstract:
Capillary zone electrophoresis (CZE) was used to monitor the whole unfolding process of bovine insulin resulted from the dithiothreitol (DTT) reduction. An off-line matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) was employed to determine the molecular weight of the reaction products at the same time to confirm the observation results obtained by CZE. The structural change during the process of bovine insulin unfolding could be observed directly from the electropherogram and information of protein unfolding could be obtained simultaneously. The results indicated that as an effective tool of monitoring the conformational change of protein,the method of CZE was simple,quick,sensitive and lower sample consumption.
