Determination of β-Galactosidase Activity by Convolution Spectrum Method
- VernacularTitle:褶合光谱法测定β-半乳糖苷酶的活性
- Author:
Hong ZHENG
;
Yutian WU
;
Huisheng FANG
- Publication Type:Journal Article
- From:
Chinese Journal of Analytical Chemistry
2001;29(5):583-585
- CountryChina
- Language:Chinese
-
Abstract:
The optimum convolution spectra of β-galactosidase and the linear equation of Qj=1.906×10-2＋4.946×10-3 C＋1.909×10-4 C2,r=1.0000 under the best condition were established by the convolution spectrum method.Based on the chromogenic substrate o-nitrophenyl-β-galactoside,a method for the determination ofβ-galactosidase activity in the COS-1 extraction sample which had been transfected transiently expression vector of β-galactosidase was determined by using the convolution curve transformation technology.The convolution spectrum method was compared with the absorption method and the result was satisfactory.The method was simple,convenient,fast and valuable to the biochemical analysis.
