Separation of Deoxyribonucleic Acid in Glucose-Hydroxy-Propylmethyl Cellulose Solution by Capillary Electrophoresis
- VernacularTitle:毛细管电泳中葡萄糖-羟丙基甲基纤维素体系分离脱氧核糖核酸
- Author:
Yan JIN
;
Bingcheng LIN
;
Yingsing FUNG
- Publication Type:Journal Article
- From:
Chinese Journal of Analytical Chemistry
2001;29(5):503-506
- CountryChina
- Language:Chinese
-
Abstract:
Glycerol,mannitol has been used as additives for the separation of deoxyribonucleic acid (DNA)fragments in entangled solution by capillary electrophoresis. Differing from glycerol and mannitol,the structure of glucose is a ring with 5 hydroxyl groups.This structure helps it easily to form complex with boric acid and hydroxypropyl methylcellulose (HPMC).On the other hand,glucose has large space obstacle compared with glycerol and mannitol.So glucose can markedly improve separation of DNA in HPMC solution by capillary electrophoresis compared with other additives.The factors such as boric acid concentration and pH,influencing formation of complex of HPMC and glucose were studied. High concentration of boric acid could improve the DNA separation in glucose-HPMC matrix system. The optimization pH to separate DNA in HPMC-glucose solution by capillary electrophoresis was 8.3.
