Insulin enhances glucose transporter 1 gene expression of low-flow ischemic myocardium
- VernacularTitle:胰岛素增强缺血心肌葡萄糖转运子1基因表达的实验研究
- Author:
Renfu YIN
;
Jinming CHEN
;
Zonggui WU
;
Shaohua QIU
;
Yongmei WANG
;
Ruimei WU
;
Xiantao KONG
- Publication Type:Journal Article
- From:
Chinese Journal of Pathophysiology
2001;17(6):531-533
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the mechanism underlying insulin-stimulated increase in glucose uptake during low-flow myocardial ischemia. METHODS: The expression of myocardial GLUT1 polypeptide was determined by semiquantitative immunoblotting. The expression of GLUT1 mRNA was determined by semiquantitative Northern blotting. RESULTS: After infusing insulin during low- flow myocardial ischemia for 8 h，the expression of both GLUT1 mRNA and GLUT1 polypeptide was significantly higher in experimental myocardium than that in normal myocardium. The glucose uptake was upregulated at the same time in the exprimental myocardium. CONCLUSION: Insulin enhances the expression of GLUT1 mRNA and GLUT1 polypeptide in ischemic myocardial regions. GLUT1 expression may be an important mechanism by which myocardial cells enhance glucose uptake and metabolism during low-flow myocardial ischemia.
