The kinetic alteration of nitric oxide formation in the lungs in the development of pulmonary fibrosis of rats
- VernacularTitle:大鼠肺纤维化形成过程中肺内一氧化氮代谢的动态变化
- Author:
Xiaoling CHEN
;
Shansheng HUANG
;
Yingmin LI
;
Wenbin LI
;
Xinliang WANG
;
Qiuhong WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Pathophysiology
2001;17(6):534-537
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the kinetic alteration of nitric oxide formation in the lungs in the development of pulmonary fibrosis in the rat. METHODS: The contents of hydroxyproline in the lungs, NO-2/NO-3 (nitrite/nitrate) in out-flowing and in-flowing pulmonary blood (OPB, IPB) were assayed on the day 7, 14, 21, 30 and 70 after intratracheal administration of bleomycin A5 . The content of NO-2/NO-3 in supernatants of culture of the alveolar macrophages (AMs) and the amount of iNOS positive stain cells in lung tissue section were also observed in the rat on 14th day after-bleomycin A5 administration. RESULTS: The content of lung hydroxyproline had no change on the 7th day, increased on the 14th day (P＜0.05), increased significantly on the 21th day, 30th day and 70th day post-bleomycin A5 compared with control rats. On the 7th day and 14th day, the content of NO-2/NO-3 increased in OPB and decreased in IPB (P＜0.01). On the 21th day, the content of NO-2/NO-3 abated in OPB (P＞0.05) but still decreased in IPB (P＜0.01). On the 30th day and the 70th day, the NO-2/NO-3 level recovered both in OPB and IPB. AMs from rats on the 14th day post-bleomycin A5 showed significant elevation (P＜0.01) in NO-2/NO-3 level. The amount of iNOS positive stain cells increased in rats on the 14th day post-bleomycin A5. CONCLUSION: The amount of NO in the lungs was high in the initial phase of fibroproliferative reaction induced by bleomycin A5 ,and these might be associated with the enhanced ability of AMs to release NO and the increased amount of iNOS.
