The Mechanisms of Arsenic Trioxide-induced Apoptosis in Human Gastric Adenocarcinoma SGC7901 Cells
- VernacularTitle:三氧化二砷诱导人胃腺癌细胞 SGC7901凋亡机制的研究
- Author:
Mao XING
;
Enjuan ZHANG
- Publication Type:Journal Article
- From:
China Pharmacy
2001;12(6):333-334
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE： To investigate the possible role of the alteration of mitochondrial transmembrane potentials(Δ Ψ m) in arsenic trioxide(As2O3)-induced apoptosis of human gastric adenocarcinoma cells(SGC7901).METHODS： Cell apoptosis was assessed through morphology， cell viability， the percentage of sub-G1 cells and phosphatidylserine(PS) externalization.the Δ Ψ m was detected on flow cytometry through double staining of Rhodamine 123 (Rh123) and propidium iodide(PI).RESULTS： The As2O3-induced apoptosis was associated closely with the disruption of the Δ Ψ m.CONCLUSION： The disruption of the Δ Ψ m is one of the mechanisms of As2O3-induced apoptosis.
