Pharmacokinetics of Pirarubicin in Patients With Breast Cancer
- VernacularTitle:吡喃霉素在乳腺癌患者体内的药代动力学
- Author:
Jianrong HE
;
Yan ZHANG
;
Jianfeng CHANG
- Publication Type:Journal Article
- From:
China Pharmacy
2001;12(6):352-353
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To estabish a HPLC assay for the determination of pirarubicin(Pir) in plasma.METHODS: Daunomycin(DM) was used as the internal standard.Plasma samples were extracted with CHCl3∶ CH3OH(2∶ 1) .0.4M NH4Cl buffer(pH=9.0) solution: CH2OH(1∶ 9) and the internal standard were added.Separation was carried out on a 250mm× 4.6mm(5μ m) phenomenex column with 0.04M KH2PO4(pH=3.0) ∶ CH3CN(68∶ 32) as mobile phase.Fluorescent detector was set at an excitation wavelength of 480nm and an emission wavelength of 550nm.RESULTS: The calibration curves for serum Pir was linear over the range of 5~1 000ng/ml(r=0.9 997) .The recovery of Pir was 95.3% .The within-day and between-day variations were less than 5% .T1/2β , CLs, Vd and AUC of Pir were(12.8± 5.9) h, (128.3± 52.6) L/(m2· h) , (1 754.3± 478.2) L/m2 and (428.7± 137.2) ng/(h· ml) , respectively.CONCLUSION: The method is suited for monitoring blood concentration and pharmacokinetic study of Pir.