Determination of Trace Selenium in Environmental Waters After Microwave Digestion and Fluorometric Determination with 2,3-Diamino-naphthalene
- VernacularTitle:利用微波消解和高效液相色谱荧光检测法测定环境水样中痕量硒
- Author:
Yuxi GAO
;
Zijian WANG
;
Chunhua LENG
- Publication Type:Journal Article
- From:
Chinese Journal of Analytical Chemistry
2001;29(6):629-632
- CountryChina
- Language:Chinese
-
Abstract:
A new approach, combining microwave digestion and sensitive determination of selenium by a normal-phase high performance liquid chromatography after derivatization with 2,3-diamino-naphthalene was proposed to the measurements of trace selenium concentration in natural waters. Two digestions systems, I.e. HNO3 and H2SO4-H2O2, were evaluated and their working conditions were optimized. Under optimal conditions, this approach provides a detection limit of 7.2 pg (with HNO3） or 10.4 pg (with H2SO4-H2O2）, a recovery of 99.1%～103.4%, as well as a linear range of 0～1.5 ng Se(Ⅳ). The approach was applied to determine Se concentration in 4 different types of environmental waters (sample volume: 2 mL) and the concentration levels of 0.20～0．90 μg/L were found, with recoveries of 99.1%～103．4% by standard addition.
