Interaction Between Quinolone Antibacterial Agents and Catalase by Fluorescence Spectroscopy
- VernacularTitle:荧光光谱法研究喹诺酮抗菌素与过氧化氢酶的相互作用
- Author:
Pinggui YI
;
Zhicai SHANG
;
Qingsen YU
- Publication Type:Journal Article
- From:
Chinese Journal of Analytical Chemistry
2001;29(6):646-648
- CountryChina
- Language:Chinese
-
Abstract:
The binding characteristics of quinolone antibacterial agent, ofloxacin (OFLX) and ciprofloxacin(CPFX) with catalase(CAT) were studied by fluorescence spectroscopy in aqueous solution. It was showed that both OFLX and CPFX had a strong ability to quench the CAT intrinsic fluorescence through a static quenching procedure. The formation constant K and the number of bind ing site n were further calculated according to the fluorescence quenching r esults.
