Substance P inhibits GABA-activated currents in bullfrog primary sensory neurons by protein kinase C
- VernacularTitle:P物质通过蛋白激酶C抑制牛蛙初级感觉神经元的GABA激活电流
- Author:
Kaihui YAO
;
Junfang WU
;
Mingli JI
;
Zhibin QIAN
;
Yunlei YANG
- Publication Type:Journal Article
- Keywords:
sensory neuron;
protein kinase C;
substance P;
γ-aminobutyric acids;
whole-cell patch-clamp technique;
bullfog
- From:
Journal of Xinxiang Medical College
2002;19(2):77-80
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the mechanism or pathway through which Substance P(SP) inhibits r-aminobutyric acid(GABA) activated currents in bullfrog dorsal root ganglion(DRG) neurons.Method The experiment was conducted on freshly isolated bullfrog dorsal root ganglion neurons using a whole-cell patch-clamp technique.Results SP could caused a slow inward current when SP was applied to DRG neurons;SP could inhibits GABA-activated currents;The inhibition could be reduced largely when protein kinase C(PKC) inhibiter,1-(5-isoquinolinesulphorry)-2-methylpiperazine(H-7), was dialyzed in cell body.Conclusion The SP ton inhibit GABA-activated currents through protein kinase C.
