Protein Components of Lewy Bodies (LBs) Identified from Synthetic Proteasome Inhibitor (PSI)-induced Inclusions in PC12 Cells by MS Analysis
- VernacularTitle:质谱鉴定PC12细胞蛋白酶体抑制剂PSI-诱导性包含体中的LB蛋白质
- Author:
Xingan LI
;
Yingjiu ZHANG
;
Yihong HU
;
Ming CHANG
;
Tao LIU
;
Danping WANG
;
Yu ZHANG
;
Linsen HU
- Publication Type:Journal Article
- Keywords:
s Lewy body;
peptide mass fingerprints;
proteasome inhibition;
protein component;
PSI-induced inclusions
- From:
Chinese Journal of Biochemistry and Molecular Biology
2008;24(10):906-915
- CountryChina
- Language:Chinese
-
Abstract:
Proteomic analysis is an effective way to identify protein constituent in Lewy bedy-like inclusions (or aggresome) in vitro. Exposure to synthetic proteasome inhibitor (PSI, 10 μmol/L) for 48 hours was used to induce the formation of cytoplasmic proteineous inclusions (termed as PSi-induced inclusions) in PC12 cells.The proteomic approaches of biochemical fractionation, two-dimensional electrophoresis (2-D) and identification via peptide mass fingerprints (PMF) were deployed, and 20 protein components of LBs were identified,i ncluding 2 proteins involved in the production of synaptic neurotransmitter, 6 subunits of the 26 S proteasome,2 cytoskeleton proteins, 2 subunits of mitochondrial complexes, 1 anti-oxidant protein, and 7 chaperone proteins and (or) chaperone-like proteins. The results suggested that these LB protein components might had been recruited in PSI-induced inclusions formed in PC12 cells under the condition of proteasome inhibition.
