Resolution of DL-Phenylalanine by Papain and Immobilized Papain
- VernacularTitle:用木瓜蛋白酶及固定化木瓜蛋白酶拆分DL-苯丙氨酸
- Author:
Ping HE
;
Zhuolie HUANG
;
Guanghong WU
;
Zhizhan CHU
;
Qingcui SHI
;
Jiale HUANG
- Publication Type:Journal Article
- Keywords:
immobilized papain;
DL-phenylalanine;
resolution;
optical purity
- From:
Chinese Journal of Biochemistry and Molecular Biology
2009;25(1):23-29
- CountryChina
- Language:Chinese
-
Abstract:
In order to separate chiral D-phenylalanine from L-phenylalanine,the enzyme catalytic method by papain and immobilized papain were used in this investigation.It indicated that the yield rate of N-acetyl-DL-phenylalanine synthesized from DL-phenylalanine was 88.7%.The ionic strength was the strongest influencing factor in the synthesis of N-acetyl-L-phenylalanylaniline by both papain and immobilized papain on sodium alginate-ehitosan(IPSAC).But the reaction temperature was the strongest influencing factor in the synthesis by immobilized papain on nylon cloth(IPN).The ionic strength and the pH were the important influencing factors in the synthesis.The yield rates of the best synthesis system of N-acetyl-L-phenylalanylaniline by papain,IPSAC,IPN were 61.2%,54.7%,36.3%,respectively.The yield rate of L-phenylalanine from N-acetyl-L-phenylalanylaniline hydrolyzation was 59.2%.The optical purity of the product was 96.6%.The vield rate of D-phenylalanine from N-acetyl-D-phenylalanine hydrolyzation was 61.7%.The optical purity was 95.7%.
