Polymerase Chain Reaction Detection for Porcine Endogenous Retrovirus in 4 Pig Cell Lines
- VernacularTitle:聚合酶链反应检测4种猪源细胞系中的内源性反转录病毒
- Author:
Maomin LU
;
Hong JIN
;
Ruichun DENG
;
Jianguo WANG
;
Shu YANG
;
Jingfeng XIONG
;
Zhuang DING
;
Jingang ZHANG
- Publication Type:Journal Article
- Keywords:
porcine endogenous retrovirus;
polymerase chain reaction;
detection;
pig cell lines
- From:
Chinese Journal of Veterinary Science
2003;23(1):1-3
- CountryChina
- Language:Chinese
-
Abstract:
Porcine endogenous retroviruses (PERV) can infect human cell in vitro, which raised widely concerns re-garding the transmission of PERV to xenograft recipients. It's essential to establish a method for detection of PERV.3 pairs of primers were synthesized according to the sequence of gag, pol and env gene of PERV. Polymerase chainreaction (PCR) and reverse transcription PCR (RT-PCR) assays were performed for detection of PERV provirusDNA and PERV specific mRNA. The results showed that provirus DNA and mRNA of PERV existed and expressedin all 4 tested cell lines. The sizes of amplified fragments are identical with the predicted. These methods may be suit-able for monitoring PERV in other cells or tissue.
