Effect of genistein on c-myc mRNA expression induced by low density lipoprotein in endothelial cells
- VernacularTitle:金雀异黄素对低密度脂蛋白诱导的内皮细胞c-myc mRNA表达的影响
- Author:
Jin LIU
;
Minghui YAO
;
Qinyan GONG
;
Rong YU
;
Yingqing LU
- Publication Type:Journal Article
- Keywords:
genistein;
lipoprotein,low density;
endothelium,vascular
- From:
Chinese Journal of New Drugs and Clinical Remedies
2003;22(1):5-8
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of genistein on c-myc mRNA expression induced by oxidized low density lipoprotein (ox-LDL) in human vascular endothelial cells (ECV304). METHODS: LDL were isolated from healthy human plasma by gradient ultracentrifugation and oxidized by CuSO4. ECV304 cells were exposed to ox-LDL 200 mg*L-1 in the presence or absence of genistein 100 μmol*L-1 for 1, 2, and 4 h in vitro. Northern blot was employed to measure c-myc mRNA levels of ECV304. RESULTS: In response to ox-LDL 200 mg*L-1, c-myc mRNA expression in ECV304 increased by 3 fold for 1h and 3.3 fold for 2 h and decreased below the control level at 4 h. Expressions of c-myc stimulated by ox-LDL in the presence of genistein 100 μmol*L-1 for 1 h and 2 h were separately 80 percent and 60 percent of that in the absence of genistein 100 μmol*L-1. CONCLUSION: Genistein can effectively inhibit c-myc mRNA expression in ECV304 induced by ox-LDL.
