Relationship between exon deletion frequency of CDKN2/P16 and pathological type,metastasis,sex in osteosarcoma
- VernacularTitle:骨肉瘤CDKN2/P16基因外显因子2缺失率与病理分型、转移能力及性别的相关性
- Author:
Yu PANG
;
Jiacan SU
;
Yifan KANG
;
Jian CHEN
;
Yongyu CHEN
- Publication Type:Journal Article
- Keywords:
osteosarcoma;
CDKN2/P16;
PCR SSCP analysis
- From:
Chinese Journal of Tissue Engineering Research
2002;6(22):3450-3451
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study on the CDKN2/P16 gene in primary osteosarcoma.Method By using molecular biological methods that inclued genome DNA extraction from paraffined tissue and PCR SSCP analysis technique, we studied alternations of CDKN2/P16 gene in 25 primary osteosarcomas.Results (1)The deletions frequency in differentiation degree of osteosarcomas was① bone brood cell, 16.7% ;② cartilage brood cell,12.5% ;③ Fiber brood cell:20% ,(P >0.05).(2)The deletion frequency in male patients was 17.6% , female patients 12.5% ,(P >0.05).(3)In early metastatic osteosarcomas the deletion rate was 33.3% ,which was significantly higher than that of the control group with the rate of 10.5% (P< 0.05).(4)The deletion rate was 16% and the mutations were not found.Conclusion (1)The deletion rate was 16% and the mutations were not found.This suggests that the deletions of CDKN2/P16 gene were closely related to the genesis of primary osteosarcoma and that the main type of the alternation of CDKN2/P16 gene was deletion.(2)In early metastatic osteosaarcomas the deletion rate was 33.3% , which was significantly higher than that of the control group with the rate of 10.5% .This indicates to great extend that the deletions of CDKN2/P16 gene were closely related to the metastatic ability.(3)The deletions frequency had no significant relationship with differentiation degree of osteosarcomas, so was with the sex of the patient.
