MOLECULAR CLONING AND MAKING OF THE cRNA PROBES OF MELATONIN RECEPTOR1a AND RETINOID-RELATED ORPHAN RECEPTORβ
- VernacularTitle:褪黑素受体Mel1a和RORβ的基因克隆和cRNA探针的制备
- Author:
Xinhui WANG
;
Jiangning ZHOU
- Publication Type:Journal Article
- Keywords:
melatonin receptors;
Mel1a;
RORβ;
pBluescript Ⅱ(KS+);
cRNA probe
- From:
Chinese Journal of Neuroanatomy
2003;19(2):145-149
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the expression of melatonin receptors Mel1a and RORβ in the human brain,and to furthe investigate the melatonin's function in Alzheimer's disease,two melatonin receptor cDNAs were cloned and introduced into pBluescript Ⅱ (KS+)vector.The recombinant plasmids were identified by using restriction enzymes and then sequenced.The results indicated that the cloned DNA sequences and the Genbandk Sequences were inentical.The cRNA probes made from them can be used in the in situ hybridization immunohistochemistry to detect the mRNA expression level in the future study.
