Application of EST Data of Siderophore Regulation Protein-like Gene of Aspergillus oryzae RIB40
- Author:
Lin XIE
;
Guizhi JU
;
Goto KUNIYASU
- Publication Type:Journal Article
- Keywords:
RNA,antisense;
siderophore regulat ion protein;
Aspergillus oryzae
- From:
Journal of Jilin University(Medicine Edition)
2003;29(1):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To acquire the new clear color phenotype of Aspergillus oryzae b y th e antisense strategy of siderophore regulation protein (SREP)-like gene. Method s: Construct the cDNA library of Aspergillus oryzae RIB40 and amplify the fr agme nt ac7336f from the every EST clone, which had high homology with SREP gene of o ther species, then construct the eukaryotic expression vector with SREP-like ge ne using antisense strategy. Results: The sequence of this SREP-like gene was a cquired, the vector was successfully constructed. Conclusion: The deduced amino acid sequence of SREP-like gene of Aspergillus oryzae indicated that there is t he high homology with those of SREP genes of Penicillium chrysogenum, Neur ospora crassa and Schizosaccharomyces pombe.
