A quantitative analysis of interactions among ethanol, chloral hydrate and naloxone
- VernacularTitle:乙醇、水合氯醛和纳洛酮3药相互作用的定量分析
- Author:
Jie LI
;
Duanzheng XU
;
Qinyan GONG
;
Minghui YAO
;
Rong YU
;
Yingqing LU
- Publication Type:Journal Article
- Keywords:
drug interactions;
multivariate analysis;
mice;
synergism;
antagonism;
isobologram;
Q-test;
multivariate logistic regression
- From:
Chinese Journal of New Drugs and Clinical Remedies
2003;22(7):387-394
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To analyse quantitatively the interactions among ethanol, chloral hydrate and naloxone with isobologram, Q-test and multiple logistic regression methods. METHODS: The hypnotic effects of the three drugs on Kunming mice were observed. In two drugs interaction study, chloral hydrate and ethanol were given at different ratios (25∶75, 50∶50, 78∶22 and 80∶20). In three drugs interactions study, 15 min after treatment of naloxone at fixed dose (0.5 mg*kg-1 and 0.2 mg*kg-1) the mixture of chloral hydrate and ethanol (at 1∶1 and 1∶3 ratio) was given to induce sleep. The ED50 for hypnotic action (righting reflex loss) of chloral hydrate, ethanol, naloxone and their mixtures were calculated by use of isobologram, interaction Q-index test and multivariate logistic regression analysis. RESULTS: The mixtures of ethanol and chloral hydrate in all ratios revealed partial but significant synergism. But in addition with naloxone the three agents showed different natures of interactions according to different naloxone levels. CONCLUSION: There are synergistic interactions in hypnotic ED50s between ethanol and chloral hydrate at different ratios and antagonistic interaction in adding a fixed dose of naloxone. The results coincide with the pharmacologistic mechanism discussed in this paper.
