Cloning of Mouse Enamel Matrix Serine Proteinase Encoding Mature Protein
- Author:
Yabing MU
;
Hongchen SUN
;
Zebing ZHANG
;
Jie OUYANG
- Publication Type:Journal Article
- Keywords:
Tooth germ;
Mice;
Serine endopeptidases;
Reverse transcriptase polymerase chain reaction;
Polymerase chain reactiton;
Cloning,molecular
- From:
Journal of Jilin University(Medicine Edition)
2003;29(6):705-709
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To clone cDNA of enamel matrix serine proteinase (EMSP1) encoding mature protein from mouse dental germs. Methods: Total RNA was isolated from developing incisors and molars of 7 days mouse pups and reverse-transcribed into cDNA. Two pairs of specific primers was designed to obtain the desired gene by Touchdown PCR and Nested PCR. The segment was inserted into Vector pMD-18T, and recombined vectors was transformed into E.coli JM109.The positive clone was chose and analysed by restriction endonuclease mapping and DNA sequencing. Results:700 bp of cDNA of mouse EMSP1 was sueccessfully cloned from mouse tooth germs tissue. The sequence was consistent with that displayed in PubMed. Conclusion:The mouse EMSP1 cDNA encoding mature protein is obtained for further study.
