Effects of sodium magnesium fructose diphosphate on free calcium concentration and nitric oxide synthase activity of ischemic synaptosome
- VernacularTitle:果糖二磷酸钠镁对缺血突触体游离钙浓度及一氧化氮合酶活性的影响
- Author:
Fanxin ZENG
;
Zhi DONG
;
Qixin ZHOU
- Publication Type:Journal Article
- Keywords:
cerebral ischemia;
sodium magnesium fructose diphosphate;
fructose-1,6-diphosphate;
synaptosome;
free calcium;
nitric oxide synthase
- From:
Acta Pharmaceutica Sinica
2003;38(5):325-327
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effects of sodium magnesium fructose diphosphate (SMFD) on free calcium concentration and nitric oxide synthase activity of ischemic synaptosome, so as to explore the protective mechanisms of SMFD on cerebral ischemia. Methods The synaptosomes from normal rat brain were prepared by phase partition and cultured with oxygen-glucose deprivation to establish ischemic synaptosome model. The intrasynaptosomal free calcium concentration and nitric oxide synthase activity were detected separtately after the synaptosomes were co-incubated with SMFD (1.3 mmol*L-1) or fructose-1,6-diphosphate (FDP, 4.0 mmol*L-1) for 60 min. Results SMFD decreased the free calcium concentration and reduced the activity of nitric oxide synthase (NOS) of ischemic synaptosomes. Its effects were more powerful than those of FDP. Conclusion SMFD may protect neurons from ischemic injury by preventing intracellular Ca2+ overload and inhibiting the activity of nitric oxide synthase.
