Experimental study of inductive apoptosis of Hep-6 in liver tumor cells line by magnetic nanoparticle encapsulated epirubicin
- VernacularTitle:纳米磁诱导人肝癌Hep-6细胞凋亡的探索性实验
- Author:
Shanxing OU
;
Xiangsheng XIAO
;
Huangwen LAI
;
Jiang XIAN
;
Jing YANG
;
Jiangtao WANG
;
Zenggui SU
- Publication Type:Journal Article
- Keywords:
magnetic nanoparticle;
apoptosis;
liver neoplasms;
epirubicin hcl
- From:
Chinese Journal of Tissue Engineering Research
2003;7(20):2812-2813
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of magnetic nanoparticleencapsulated epirubicin(MNPE) on inducing apoptosis of human liverHep-6 tumor cell line in vitro and provide new method for local ablation ofliver in order to improve survival period of patients and quality oflife. Methods Inductive apoptosis of nano-magnetic pharmoparticle toHep-6 tumor cell of primonary hepatic cell caner was investigated by DNAelectrophoresis, electron nicroscopy , and flow cytometry analysis. Theseitems were divided into three groups, control, drug-control, and grouptreated with magnetic nauoparticle encapsulated epirubicin. The changes ofhuman liver Hep-6 apoptosis induced by magnetic nanoparticle encapsu-lated epirubicin were observed on different time-point and with differentnanoparticle encapsulated epirubicin and control group of biaoroubixingwere divided into high-dosage and low-dosage group. And the ultimateconcentration of 10 mg/L and 100 mg/L were given respectively on hu-group was iucreased from 25% to 54% after 24 hours. The apoptosis ratein the experimental group, biaoroubixing group and control group was78%, 53% and 2% respectively after 36 hours. There was significantdifference( t = 3.05. P ＜ 0.05) between the results of each group. Theapoptosis rate and quantity of medicine presented positive relativity withtime ( r = 0.96, P ＜ 0.05 ) .Conclusion Magnetic nanopartiele encap-sulated epirubicin presents the advantages of slow degradation, release ofmagnetic nanoparticle system and better target and can induce apoptosis ofliver tumor Hep cell.
