Construction of cDNA library of the treated Changliver cell and quality analysis
- VernacularTitle:常氏肝癌细胞cDNA文库的构建及质量分析
- Author:
Juntang LIN
;
Congrui WANG
;
Huiyong ZHANG
;
Yuchang LI
;
Cunshuan XU
- Publication Type:Journal Article
- Keywords:
cDNA library;
switching mechanism at 5′end of RNA transcript;
Changliver cell;
long-distance PCR;
immunoscreening
- From:
Journal of Xinxiang Medical College
2004;21(1):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct cDNA library of the treated Changliver cell by switching mechanism at 5′ end of RNA transcript (SMART) technique and analyze its quality.Methods cDNA of Changliver cell was aquired with reverse transcription polymerase chain reaction (RT-PCR) and long-distance PCR (LD-PCR),then the cDNA library was constructed with SMART cDNA library construction kit.Results Through testing,the high quality cDNA library containing full length cDNA of Changliver cell had been constructed.The titer of the amplified cDNA library was 4.5 × 1010 pfu*ml-1 and the average exogenous inserts of the recombinants was 1.5 kb.Conclusion These results suggest that the Changliver cell cDNA library has a high quality and lays a solid foundation for researching on Changliver cell and screening
