The posibility study of human mesenchymal stem cells harvested and cultivated in vitro as seeds cells for reconstruction of throat and trachea cartilage tissue engineering
- VernacularTitle:人骨髓间充质干细胞的获取与体外培养的研究 : 作为喉、气管软骨组织工程重建种子细胞的可能性
- Author:
Xun JIANG
;
Pengcheng CUI
;
Wenxian CHEN
;
Dianzhong ZHANG
;
Zhipei ZHANG
;
Daqing ZHAO
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2001;5(22):146-147
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the feasibility of using bone marrow-derived mesenchymal stem cells (MSCs) as seed cells for tissue-engineering in Otolaryngology. Method MSCs were isolated from bone marrow of human rib and purified by centrifuge and cultured in vitro. The proliferation and growth characteristics of MSCs were observed in primary and passage culture. Result Human bone marrow-derived MSCs showed active proliferation capacity in vitro in primary and passage cultures. Conclusion Human bone marrow-derived MSCs have relatively young biologic age and they can be used as the seed cells for tissue engineering.
