Effects of isoliensinine on proliferation of porcine coronary arterial smooth muscle cells induced by phenylephrine
- VernacularTitle:异莲心碱对苯肾上腺素诱导猪冠脉平滑肌细胞增殖的影响
- Author:
Junhua XIAO
;
Yanlin ZHANG
;
Lili DING
;
Xiuling FENG
;
Jialing WANG
- Publication Type:Journal Article
- Keywords:
isoliensinine;
phenylephrine;
cell proliferation;
protooncogene;
heat shock protein 70
- From:
Acta Pharmaceutica Sinica
2005;40(2):105-110
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the inhibitory effects and mechanism of action of isoliensinine (IL) on the proliferation of porcine coronary arterial smooth muscle cells (CASMCs) induced by phenylephrine (Phen) and its mechanisms of action. Methods MTT assay, immunohistochemical method and Western blotting were adopted. Results IL (0.03-3 μmol·L-1) could inhibit the CASMCs proliferation induced by Phen (0.1 μmol·L-1) in a concentration-dependent manner. IL (0.1 μmol·L-1) antagonized Phen-induced overexpression of PDGF-β and bFGF from 0.545±0.026 and 0.47±0.03 to 0.458±0.019 and 0.376±0.017 (P<0.01, P<0.01). IL (0.1 μmol·L-1) also decreased c-fos, c-myc and hsp70 overexpression induced by Phen from 0.57±0.04, 0.44±0.04 and (173±36)% to 0.46±0.05, 0.372±0.021 and (115±35)% respectively (P<0.01, P<0.01, P<0.01). Conclusion IL exerted antiproliferative effect on CASMCs induced by phenylephrine, and its mechanisms were related to decrease the overexpression of growth factors (PDGF-β, bFGF), protooncogene (c-fos, c-myc) and hsp70.
