Expression of Bovine Interleukin-2 Gene in Pichia pastoris
- VernacularTitle:牛白细胞介素-2在毕赤酵母中的表达
- Author:
Fang CHEN
;
Hongli SUN
;
Xiangrong CAO
;
Zhen LI
;
Ruisong YU
- Publication Type:Journal Article
- Keywords:
bovine Interleukin-2;
Pichia pastoris;
gene expression;
pPICZB
- From:
Chinese Journal of Veterinary Science
2005;25(2):178-182
- CountryChina
- Language:Chinese
-
Abstract:
The Interleukin-2 gene cDNA was cloned into the Pichia pastoris expression vector pPICZB,which is under the control of the alcohol oxidase promoter AOX1. The linearized recombinant plasmid of BoIL2-pPICZB,digested by Sac I ,was transformed into X-33 strains by electroporation. The multi-copy insert transformants were screened by Zeocin-resistance and induced by 1% methanol. The intracellular expression products were tested by SDS-PAGE analysis and Western blotting. Purified recombinant BoIL2 was gained by metal-chelating affinity chromatographic (MCAC). Assay with murine CTLL-2 cells showed that the recombinant BoIL2 exhibited the biological activity.
